Download Atlas of Confocal Laser Scanning In-vivo Microscopy in by Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, PDF

By Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, Phd, Prof.Dr. rer. nat. Joachim Stave (auth.)

Confocal microscopy with laser scanning expertise yields in-vivo pictures of ocular and ocular adnexal surfaces which are so incredible that they rival histology when it comes to quality.This certain atlas and textbook demonstrates basic in-vivo anatomy of the cornea, limbus and conjunctiva, quantifies a variety of mobile buildings utilizing cell-density calculations and establishes correlations among novel optical sections of varied ailments of the ocular floor and scientific findings. moreover, it helps the translation of novel high-magnification optical sections via evaluating corneal and conjunctival imprint cytology with in-vivo photos and describes early inflammatory alterations in corneal grafts, in addition to corneal conjunctivalisation in limbal stem mobile deficiency, corneal dystrophies or infections, flap interface and margin features after laser in-situ keratomileusis (LASIK). furthermore, it instructs the reader approximately diagnostic and healing follow-up recommendations and gives a short advent to functions in different fields corresponding to dentistry and ear, nostril and throat surgery.

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Extra resources for Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophthalmology

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4 Ex Vivo Applications Ex vivo confocal imaging using complex microscope systems has become a prominent and integral feature of modern-day scientific technology. Ex vivo conditions enable the process of image acquisition to be optimized and standardized, thus yielding optimal resolution and contrast. The information obtained in this way may be helpful when similar structures have to be identified and interpreted under in vivo conditions. The following examples illustrating blood constituents, pathogenic microorganisms, and ocular tissue structures demonstrate the resolution of the RCM under optimized experimental conditions.

1), enables the fine structure of the tear film to be imaged (Fig. 2). The rapid imaging sequence in the device also permits dynamic processes to be recorded [51, 77, 88]. a Fig. 3 Normal and pathological tear film. a Normal tear film, noncontact examination. 2 Pathological Findings b up time; the surface of the superficial cells is visible in the area of the dry spot b Fig. 1 Superficial Cells (Up to Approximately 50 mm in Diameter) In the case of the most superficial epithelial cells, bright cell borders and a dark cell nucleus and cytoplasm are readily visualized on confo- cal laser scanning microscopy.

34 Calcific band keratopathy. 2 Epithelium Fig. 35 Epithelial basement membrane dystrophy (map-dot-fingerprint dystrophy) with fingerprintlike corneal lesions. a Slit-lamp photograph of fingerprint lesions. b–d Linear hyperreflective structures corresponding to abnormal basement membrane insinuated into the corneal epithelium. e Oblique section clearly showing this aberrant tissue within the corneal epithelium a b c d e 51 52 Chapter 5 Confocal Laser Scanning In Vivo Microscopy a Fig. 36 Epidemic keratoconjunctivitis.

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