By S. Mohan Jain (ed.), S. K. Sopory (ed.), R. E. Veilleux (ed.)
Since the start of agricultural construction, there was a continuing attempt to develop extra and higher caliber nutrition to feed ever expanding popula tions. either stronger cultural practices and stronger crop crops have allowed us to divert extra human assets to non-agricultural actions whereas nonetheless expanding agricultural creation. Malthusian inhabitants predictions proceed to alarm agricultural researchers, in particular plant breeders, to hunt new applied sciences that would proceed to permit us to supply extra and higher nutrition via fewer humans on much less land. either development of current cultivars and improvement of latest high-yielding cultivars are universal objectives for breeders of all plants. In vitro haploid construction is likely one of the new applied sciences that convey nice promise towards the target of accelerating crop yields by means of making comparable germplasm to be had for lots of vegetation that used to be used to enforce one of many maximum plant breeding good fortune tales of this century, i. e. , the improvement of hybrid maize by means of crosses of inbred strains. one of many major purposes of anther tradition has been to provide diploid homozygous natural strains in one iteration, hence saving many generations of backcrossing to arrive homozygosity via conventional ability or in plants the place self-pollination isn't really attainable. simply because doubled haploids are similar to inbred strains, their price has been preferred by means of plant breeders for many years. the hunt for traditional haploids and strategies to urge them has been ongoing because the starting of the 20 th century.
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Extra resources for In Vitro Haploid Production in Higher Plants, Volume 4: Cereals
1994). For breeding purposes, haploid plants must be diploidized for restoration of fertility. , 1991). 16. Variation and inheritance of traits in regenerated plants Anther-derived plants are identified as the first generation of pollen plants (H 1). Their immediate progeny are known as H 2 . The genotype may not be well-expressed in the H1 generation but there is no expected genotypic difference between an H 1 plant and its H 2 generation. Segregation among the anther-derived progeny of F 1 hybrids includes plants that resemble the male and female parents of the hybrids, intermediate forms, and new types.
Plant age, photoperiod, light intensity, temperature, season, growing conditions, and nutritional status, also affect the anther culture response. Hu et al. (1981) obtained best anther culture response when plants were grown under bright sun at 18-20°C. Raina et al. 1°C. , 1992). Callus induction rate was highest when donor plants were grown under mid or short photoperiod and at mid or low temperatures. 7°C under 14 h photoperiod. The ratio of green to albino plantlets was also higher under a short photoperiod.
Plant Cell Rep. 5: 215-218. T. J. Zapata (1987) Position of anthers at plating and its influence on anther callusing in rice. Plant Cell Rep. 6: 318-319. D. S. Khush (1985) Inheritance of callus formation ability in anther cultures of rice, Oryza sativa L. Theor. Appl. Genet. 70: 113-116. , H. Suzuki, K. Hattori & Y. Futsuhara (1994) Identification of ploidy level of the regenerated plants by anther culture in rice. Breeding Sci. 44: 19-22. Narasimman, R. S. ). Euphytica 66: 19-25. S. Gosal et al.